Please use this identifier to cite or link to this item: http://repositorio.uptc.edu.co/handle/001/2499
Title: Obtención de ácido 5-Hidroximetil-2-Furancarboxílico (HMFCA) a partir de 5-Hidroximetilfurfural (5-HMF) con microorganismos aislados de bagazo de caña
Authors: Muñoz Castiblanco, Deysi Tatiana
metadata.dc.contributor.role: Martínez Zambrano, José Jobanny (Director de tesis)
Rojas Sarmiento, Hugo Alfonso (Codirector de tesis)
Keywords: Industria biotecnológica
Microbiología industrial
Control de procesos biotecnológicos
Energía biomásica
Maestría en Química - Tesis y disertaciones académicas
Issue Date: 2018
Publisher: Universidad Pedagógica y Tecnológica de Colombia
Citation: Muñoz Castiblanco, D. T. (2018). Obtención de ácido 5-Hidroximetil-2-Furancarboxílico (HMFCA) a partir de 5-Hidroximetilfurfural (5-HMF) con microorganismos aislados de bagazo de caña. (Tesis de maestría). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2499
Abstract: El ácido 5-hidroximetil-2-furancarboxílico (HMFCA) es importante como monómero en la síntesis de diversos poliésteres y tiene posible actividad antitumoral. Se obtiene por la oxidación selectiva del grupo formilo del 5-hidroximetilfurfural (5-HMF). Sin embargo, la obtención de HMFCA por métodos químicos convencionales presenta varias desventajas, como la gran cantidad de hidróxido de sodio usado y las altas temperaturas, lo cual, a nivel industrial, representa una importante cantidad de contaminantes y altos costos en su producción. Además, la utilización de catalizadores sólidos con metales de alto valor, hace que el proceso de síntesis de HMFCA sea costoso. Como respuesta a esta problemática, se han utilizado métodos biotecnológicos de síntesis menos contaminantes y económicamente sustentables como las fermentaciones, con las cuales es posible obtener productos de alto valor agregado, como el HMFCA, cuando se optimizan las condiciones para el crecimiento de los microorganismos. En la presente investigación, partiendo de residuos de bagazo de caña se aisló una cepa bacteriana capaz de degradar 5-HMF y convertirlo selectivamente en HMFCA. De acuerdo con la secuenciación del gen ribosomal 16S, la cepa bacteriana pertenece a la especie Serratia marcescens. La oxidación de 5-HMF a HMFCA se llevó a cabo en fermentaciones con las células completas. El seguimiento de la transformación de 5-HMF se realizó por cromatografía de líquidos de alta resolución (HPLC) y el crecimiento bacteriano fue determinado por espectrofotometría UV-Vis. Se utilizaron dos fuentes de 5-HMF, una correspondiente a 5-HMF puro (5-HMFp) y la otra más económica, obtenida a partir de hidrolizados de fructosa, utilizando Nb2O5 como catalizador (5-HMFf). Se determinó el nivel de tolerancia de Serratia marcescens a diferentes concentraciones de 5-HMFp y 5-HMFf. Se demostró que la bacteria fue capaz de metabolizar una concentración de 5-HMFf de 10 mM, a 30 °C y pH 8, obteniéndose un rendimiento hacia HMFCA del 78 % en 12 horas de reacción. Posteriormente, se evaluaron las condiciones de reacción: temperatura, pH y concentración de sustrato, en el rendimiento de HMFCA a partir de 5-HMFf con el uso del diseño Box-Behnken. Los resultados mostraron rendimientos por encima del 60% para HMFCA a 30 °C, pH 8, y una concentración de 5-HMF igual a 3 mM. Mediante la reacción en cadena de la polimerasa (PCR), se confirmó la presencia de la familia piridina nucleótido disulfuro oxidorreductasas en S. marcescens, como posible gen responsable de la transformación de 5-HMF a HMFCA. Asimismo, se usó la estrategia Fed-batch con control de la concentración de 5-HMFf, para obtener una mayor concentración del compuesto de interés en el medio de cultivo, llegando a obtener una concentración final de HMFCA de 790 mg L-1, usando la fuente más económica de 5-HMF, sin microorganismos modificados genéticamente, y en 20 horas de reacción.
Description: 1 recurso en línea (71 páginas) : ilustraciones color, figuras, tablas.
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