Obtención de ácido 5-Hidroximetil-2-Furancarboxílico (HMFCA) a partir de 5-Hidroximetilfurfural (5-HMF) con microorganismos aislados de bagazo de caña
| dc.contributor.advisor | Martínez Zambrano, José Jobanny | spa |
| dc.contributor.advisor | Rojas Sarmiento, Hugo Alfonso | spa |
| dc.contributor.author | Muñoz Castiblanco, Deysi Tatiana | |
| dc.contributor.financer | COLCIENCIAS | |
| dc.date.accessioned | 2019-03-28T14:50:19Z | |
| dc.date.available | 2019-03-28T14:50:19Z | |
| dc.date.issued | 2018 | |
| dc.description | 1 recurso en línea (71 páginas) : ilustraciones color, figuras, tablas. | spa |
| dc.description.abstract | 5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) is important as a monomer in the synthesis of various polyesters and has possible antitumor activity. It is obtained by the selective oxidation of the formyl group from 5-hydroxymethylfurfural (5-HMF). However, obtaining HMFCA by conventional chemical methods has several disadvantages, such as the large amount of sodium hydroxide used and high temperatures, which, at an industrial level, represents a significant amount of pollutants and high production costs. In addition, the use of solid catalysts with high value metals makes the HMFCA synthesis process expensive. In response to this problem, biotechnological methods of synthesis that are less polluting and economically sustainable have been used, such as fermentations, with which it is possible to obtain products with high added value, such as HMFCA, when the conditions for the growth of microorganisms are optimized. In the present investigation, starting from cane bagasse residues, a bacterial strain capable of degrading 5-HMF and selectively converting it into HMFCA was isolated. According to the sequencing of the 16S ribosomal gene, the bacterial strain belongs to the species Serratia marcescens. The oxidation of 5-HMF to HMFCA was carried out in fermentations with whole cells. The follow-up of the 5-HMF transformation reaction was performed by high-resolution liquid chromatography (HPLC) and bacterial growth was determined by UV-Vis spectrophotometry. Two sources of 5-HMF were used, one corresponding to pure 5-HMF (5-HMFp) and the other more economical, obtained from fructose hydrolysates (5-HMFf)., using Nb2O5 as a catalyst. The tolerance level of Serratia marcescens was determined at different concentrations of 5-HMFp and 5-HMFf. It was demonstrated that the bacteria was able to metabolize a concentration of 5-HMFf of 10 mM, at 30 ° C and pH 8, obtaining a yield towards HMFCA of 78% in 12 hours of reaction. Subsequently, the reaction conditions were evaluated: temperature, pH and substrate concentration, in the yield to HMFCA from 5-HMFf with the use of the Box-Behnken design. The results showed yields above 60 % for HMFCA at 30 °C, pH 8, and a concentration of 5-HMF equal to 3 mM. Through the polymerase chain reaction (PCR), the presence of the family pyridine nucleotide disulfide oxidoreductases in S. marcescens was confirmed as a possible gene responsible for the transformation of 5-HMF to HMFCA. Also, the Fed-batch strategy with control of 5-HMFf concentration was used to obtain a higher concentration of the compound of interest in the culture medium, reaching a final HMFCA concentration of 790 mg L-1, using the most economical source of 5-HMF, without genetically modified microorganisms, and in 20 hours of reaction. | eng |
| dc.description.abstract | El ácido 5-hidroximetil-2-furancarboxílico (HMFCA) es importante como monómero en la síntesis de diversos poliésteres y tiene posible actividad antitumoral. Se obtiene por la oxidación selectiva del grupo formilo del 5-hidroximetilfurfural (5-HMF). Sin embargo, la obtención de HMFCA por métodos químicos convencionales presenta varias desventajas, como la gran cantidad de hidróxido de sodio usado y las altas temperaturas, lo cual, a nivel industrial, representa una importante cantidad de contaminantes y altos costos en su producción. Además, la utilización de catalizadores sólidos con metales de alto valor, hace que el proceso de síntesis de HMFCA sea costoso. Como respuesta a esta problemática, se han utilizado métodos biotecnológicos de síntesis menos contaminantes y económicamente sustentables como las fermentaciones, con las cuales es posible obtener productos de alto valor agregado, como el HMFCA, cuando se optimizan las condiciones para el crecimiento de los microorganismos. En la presente investigación, partiendo de residuos de bagazo de caña se aisló una cepa bacteriana capaz de degradar 5-HMF y convertirlo selectivamente en HMFCA. De acuerdo con la secuenciación del gen ribosomal 16S, la cepa bacteriana pertenece a la especie Serratia marcescens. La oxidación de 5-HMF a HMFCA se llevó a cabo en fermentaciones con las células completas. El seguimiento de la transformación de 5-HMF se realizó por cromatografía de líquidos de alta resolución (HPLC) y el crecimiento bacteriano fue determinado por espectrofotometría UV-Vis. Se utilizaron dos fuentes de 5-HMF, una correspondiente a 5-HMF puro (5-HMFp) y la otra más económica, obtenida a partir de hidrolizados de fructosa, utilizando Nb2O5 como catalizador (5-HMFf). Se determinó el nivel de tolerancia de Serratia marcescens a diferentes concentraciones de 5-HMFp y 5-HMFf. Se demostró que la bacteria fue capaz de metabolizar una concentración de 5-HMFf de 10 mM, a 30 °C y pH 8, obteniéndose un rendimiento hacia HMFCA del 78 % en 12 horas de reacción. Posteriormente, se evaluaron las condiciones de reacción: temperatura, pH y concentración de sustrato, en el rendimiento de HMFCA a partir de 5-HMFf con el uso del diseño Box-Behnken. Los resultados mostraron rendimientos por encima del 60% para HMFCA a 30 °C, pH 8, y una concentración de 5-HMF igual a 3 mM. Mediante la reacción en cadena de la polimerasa (PCR), se confirmó la presencia de la familia piridina nucleótido disulfuro oxidorreductasas en S. marcescens, como posible gen responsable de la transformación de 5-HMF a HMFCA. Asimismo, se usó la estrategia Fed-batch con control de la concentración de 5-HMFf, para obtener una mayor concentración del compuesto de interés en el medio de cultivo, llegando a obtener una concentración final de HMFCA de 790 mg L-1, usando la fuente más económica de 5-HMF, sin microorganismos modificados genéticamente, y en 20 horas de reacción. | spa |
| dc.description.degreelevel | Maestría | spa |
| dc.description.degreename | Magíster en Química | spa |
| dc.description.notes | Bibliografía: páginas 67-71. | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.identifier.citation | Muñoz Castiblanco, D. T. (2018). Obtención de ácido 5-Hidroximetil-2-Furancarboxílico (HMFCA) a partir de 5-Hidroximetilfurfural (5-HMF) con microorganismos aislados de bagazo de caña. (Tesis de maestría). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/2499 | spa |
| dc.identifier.uri | http://repositorio.uptc.edu.co/handle/001/2499 | |
| dc.language.iso | spa | spa |
| dc.publisher | Universidad Pedagógica y Tecnológica de Colombia | spa |
| dc.publisher.faculty | Facultad de Ciencias, Escuela de Posgrados. Maestría en Química | spa |
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| dc.rights | Copyright (c) 2018 Universidad Pedagógica y Tecnológica de Colombia | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.coar | http://purl.org/coar/access_right/c_abf2 | spa |
| dc.rights.creativecommons | Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) | spa |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | spa |
| dc.subject.armarc | Industria biotecnológica | |
| dc.subject.armarc | Microbiología industrial | |
| dc.subject.armarc | Control de procesos biotecnológicos | |
| dc.subject.armarc | Energía biomásica | |
| dc.subject.armarc | Maestría en Química - Tesis y disertaciones académicas | |
| dc.title | Obtención de ácido 5-Hidroximetil-2-Furancarboxílico (HMFCA) a partir de 5-Hidroximetilfurfural (5-HMF) con microorganismos aislados de bagazo de caña | spa |
| dc.type | Trabajo de grado - Maestría | spa |
| dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | spa |
| dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
| dc.type.content | Text | spa |
| dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
| dc.type.redcol | https://purl.org/redcol/resource_type/TM | spa |
| dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
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