Regeneración adventicia a través del cultivo de anteras en papa amarilla diploide (solanum tuberosum grupo phureja)
dc.contributor.advisor | Pacheco Maldonado, José Constantino | |
dc.contributor.advisor | Rodríguez Molano, Luis Ernesto | |
dc.contributor.advisor | Arias Moreno, Diana Marcela | |
dc.contributor.author | Araque Barrera, Eyda Johanna | |
dc.date.accessioned | 2021-08-18T16:19:02Z | |
dc.date.available | 2021-08-18T16:19:02Z | |
dc.date.issued | 2020 | |
dc.description.abstract | Spa: La papa amarilla diploide conocida como “papa criolla” (Solanum tuberosum Grupo Phureja), es cultivada en la región oriental de los Andes en altitudes de 2000–3400 m, se caracteriza por presentar ciclo corto y ausencia de periodo de reposo. En Colombia, la papa criolla constituye el sustento y fuente de recursos económicos de numerosas familias que habitan las zonas rurales. Los materiales silvestres y comerciales del grupo Phureja son clones altamente heterocigóticos por lo que presentan alta diversidad genética para caracteres de interés agronómico y nutricional aún no explorados, que pueden ser utilizados en programas de fitomejoramiento. Durante más de 100 años, los estudios genéticos de la papa se han visto limitados por dos aspectos de su biología, la poliploidía y la auto-incompatibilidad los cuales restringen su reproducción, rendimiento y producción. Por tanto, es pertinente crear y/o aplicar biotecnologías, y metodologías alternativas a las tradicionales que permitan mejorar y aprovechar variantes genéticas de interés en favor de optimizar la productividad y la rentabilidad del cultivo de papa. Considerando lo anterior, se planteó como objetivo inducir regeneración adventicia a partir de cultivo in vitro de anteras en papa amarilla diploide (Solanum tuberosum grupo Phureja). Racimos de botones florales de 0.2 – 2.0 cm fueron escindidos y clasificados de acuerdo tamaño y al desarrollo de las microsporas de las anteras. Los botones florales fueron desinfectados con NaClO, se escindieron las anteras y se sembraron medio MS sin reguladores de crecimiento y suplementado diferentes fitohormonas en diferentes concentraciones. Para conocer el estado de desarrollo de las células de las anteras cultivadas in vitro, se extrajo una antera de cada tratamiento y se le realizó un raspado a su interior en una gota de Orceína Acética. Enseguida se observó el micropreparado en un microscopio óptico, se realizó registro fotográfico, y se determinó el tipo de célula y el número de núcleos de las células en división. Finalmente, y con apoyo de redes neuronales artificiales se realizó una clasificación de botones florales para determinar aquellos botones que pueden desarrollar procesos organogénicos y/o embriogénicos. Como resultado del trabajo de investigación se evidenció que el tamaño del botón floral presenta relación con la etapa de desarrollo de las células de la antera. Así como que las condiciones fisicoquímicas indujeron cambios morfológicos en las anteras cultivadas, estimularon la producción de callo y regeneración de embriones somáticos, y que en los casos que hubo dicha regeneración, las células mostraron una división celular asimétrica. Los resultados obtenidos constituirán un medio rápido para obtención de líneas totalmente homocigotas de gran valor utilizables como progenitores de cultivares F1 en programas de mejoramiento. Lo anterior, contribuirá a los procesos de selección fenotípica para características cualitativas y cuantitativas de manera más eficiente, beneficiando a todos los actores de la cadena de la papa. | spa |
dc.description.degreelevel | Maestría | spa |
dc.description.degreename | Magister en Ciencias Biológicas | spa |
dc.format.extent | 1 recurso en línea (164 páginas) : ilustraciones, tablas, figuras. | spa |
dc.format.mimetype | application/pdf | spa |
dc.identifier.citation | Araque Barrera, E. J. (2020). Regeneración adventicia a través del cultivo de anteras en papa amarilla diploide (solanum tuberosum grupo phureja). (Tesis de maestría). Universidad Pedagógica y Tecnológica de Colombia, Tunja. http://repositorio.uptc.edu.co/handle/001/3682 | spa |
dc.identifier.uri | http://repositorio.uptc.edu.co/handle/001/3682 | |
dc.language.iso | spa | spa |
dc.publisher | Universidad Pedagógica y Tecnológica de Colombia | spa |
dc.publisher.faculty | Facultad Ciencias | spa |
dc.publisher.place | Tunja | spa |
dc.publisher.program | Maestría en Ciencias Biológicas | spa |
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dc.rights | Copyright (c) 2019 Universidad Pedagógica y Tecnológica de Colombia | spa |
dc.rights | Atribución-NoComercial-SinDerivadas 2.5 Colombia | * |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_abf2 | spa |
dc.rights.creativecommons | Licencia Creative Commons Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) | spa |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.5/co/ | * |
dc.subject.agrovocuri | Papa criolla: Solanum phureja | |
dc.subject.armarc | Citogenética vegetal | |
dc.subject.armarc | Células germinales | |
dc.subject.armarc | Germoplasma vegetal | |
dc.subject.armarc | Cultivo de células vegetales | |
dc.subject.armarc | Maestría en Ciencias Biológicas - Tesis y disertaciones académicas | |
dc.subject.proposal | Anteras | spa |
dc.subject.proposal | Callo | spa |
dc.subject.proposal | Cultivo in vitro, | spa |
dc.subject.proposal | Cultivo de anteras | spa |
dc.subject.proposal | Embriogénesis | spa |
dc.subject.proposal | Organogénesis | spa |
dc.subject.proposal | Papa criolla y regeneración. | spa |
dc.title | Regeneración adventicia a través del cultivo de anteras en papa amarilla diploide (solanum tuberosum grupo phureja) | spa |
dc.type | Trabajo de grado - Maestría | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TM | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dcterms.audience | Investigadores | spa |
dcterms.audience | Docentes | spa |
dcterms.audience | Estudiantes | spa |
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